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| 产品名称： | BT-B |
|---|---|
| 商品货号： | TS259210 |
| <strong>Cell line </strong>： | BT-B |
| DSMZ no. ： | TS259210 |
| Species ： | confirmed as human with IEF of AST, NP, PEP B |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | these cells were thought to be "stablished from the malignant urinary bladder carcinoma of a 66-year-old Caucasian man (stage pTa G3) in 1989"; same donor as for cell line BT-A; however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA |
| Reference(s) ： | 15037, 15053, 15197 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 43 hours |
| Harvest ： | cell harvest of about 0.2-0.3 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 13% polyploidy - 72(68-74)<3n>XX, -Y, +1, -4, +5, +5, +5, -11, +12, +12, -19, -21, -22, +3mar, der(1)t(1;3)(p11;q11), der(1)t(1;5) (p21;q21), der(2)t(2;5)(p11;q11), del(3)(q13), add(4)(q35), del(5)(p12), i(5p)x3, add(6)(q25/27), i(7q), del(8)(q23), del(10)(p11), inv(12)(q21q24)/der(12)t(12;?)(q21;?)x2, add(19)(p13) - mar1 resembles i(Xp) - HELA markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV +, SMRV - |