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| 产品名称： | SBC-2 |
|---|---|
| 商品货号： | TS259214 |
| <strong>Cell line </strong>： | SBC-2 |
| DSMZ no. ： | TS259214 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | these cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-7 (DSM ACC 229); however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA |
| Reference(s) ： | 14396, 15014 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers, often triangle-formed cells |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:10 to 1:20 every second day using trypsin/EDTA; seed out at about 1 x 106 cells/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | cell harvest of about 20-25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 2% polyploidy - 75(66-80)<3n>XX, -X, +1, +3, -4, +5, +6, +8, -9, +10, +11, +11, +12, -13, -14, -15, +16, +17, -18, -22, +3-5mar, del(3)(q11), der(3)t(1;3)(q11;q13), der(3)t(3;5)(q13;q11), i(5p), i(7p)x1-2, der(8)t(8;11)(q24;q11), der(9)t(9;11)(p21;q11), i(11p)x3, add(12)(?q21), add(15)(p11), del(17)(p12) - karyotype closely related to SBC-7 - HELA markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |