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| 产品名称： | SBC-7 |
|---|---|
| 商品货号： | TS259215 |
| <strong>Cell line </strong>： | SBC-7 |
| DSMZ no. ： | TS259215 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | cervix carcinoma (derivative of HELA) |
| Origin ： | these cells were thought to be "established from the malignant urinary bladder carcinoma of a 82-year-old Caucasian woman (stage pTa G1-G2) in 1992"; same donor as for cell line SBC-2 (DSM ACC 228); however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of HELA |
| Reference(s) ： | 14396, 15014 |
| Biosafety level ： | 1 |
| Risk assessment ： | The cell line is infected with papilloma virus type 18 (HPV-18). The cells contain several copies as proviruses integrated into the eukaryotic genome. The integrated virus genomes are incomplete and exhibit 2-3 kb deletions of the E2-L2 region. An activation and transmission of the HPV-18 during handling of the cell line is improbable. The cell line is categorized biosafety level 1. |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | split confluent culture 1:5 to 1:10 about twice a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 15-17 hours |
| Harvest ： | cell harvest of about 1-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as HELA using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human hypertriploid karyotype with 15% polyploidy - 73/74(70-77)<3n>XX/XXX, +1, -4, +5, +6, -9, -9, +11, -13, -15, +16, +16, +17, +17, -22, -22, +4mar, der(1)t(1;14)(p11;q11), i(3q), del(3)(q11), add(5)(p11), i(7q), der(8)t(8;?11)(q24;q11), i(11p)x2, add(15)(p11), del(17)(p12)x2 - HELA markers present |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/II -, MLV -, SMRV - |