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| 产品名称： | BL-70 |
|---|---|
| 商品货号： | TS259221 |
| <strong>Cell line </strong>： | BL-70 |
| DSMZ no. ： | TS259221 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | Burkitt lymphoma |
| Origin ： | established from the tumor tissue at relapse of a 16-year-old Caucasian boy with Burkitt lymphoma; cells were described in the literature to be EBV-negative |
| Reference(s) ： | 14434, 14714 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells growing non-adherent in suspension |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 µM mercaptoethanol) |
| Subculture ： | optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at ca. 0.3 x 106 cells/ml; start culture with 20% FBS after thawing |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 44 hours |
| Harvest ： | saturation density of about 1.0 x 106cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 4% polyploidy - 47(42-49)<2n>XY, +7, inv(1)(p21q21), del(2)(q33), t(8;14)(q24;q32), t(12;22)(q21.1;q13.2) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |