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| 产品名称： | A-427 |
|---|---|
| 商品货号： | TS259224 |
| <strong>Cell line </strong>： | A-427 |
| DSMZ no. ： | TS259224 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | lung carcinoma |
| Origin ： | established from the lung carcinoma of a 52-year-old Caucasian man |
| Reference(s) ： | 14556, 14861 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture ： | split confluent culture 1:3 every 4-5 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2; after thawing, recovery may be slow and adherence may be reduced |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60-70 hours |
| Harvest ： | cell harvest of about 25 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near triploid karyotype with 2% polyploidy - 67(59-72)<3n>XXY, +3, +7, -13, -14, -15, -18, -19, -20, -22, +4mar, add(1)(p11), del(1)(q11), del(3)(p11)x2, der(3)t(1;3)(q21;q11), add(4)(q31), del(6)(q21), del(7)(p11), i(10q), add(11)(q24), add(14)(p11), add(15)(p13), add(18)(q23) - 3p- deletion is characteristic of lung carcinoma - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |