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| 产品名称： | IGR-1 |
|---|---|
| 商品货号： | TS259228 |
| <strong>Cell line </strong>： | IGR-1 |
| DSMZ no. ： | TS259228 |
| Species ： | confirmed originally as human with IEF of AST, NP and later by STR fingerprinting |
| Cell type ： | melanoma |
| Origin ： | established from the metastatic tumor in a groin lymph node of a 42-year-old man with malignant melanoma (primary tumor histology: NM) |
| Reference(s) ： | 14363 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing as monolayers (dendritic branching cells until confluence, upon contact inhibition smooth and round); image; image |
| Medium ： | 85-90% Dulbeccos MEM + 10-15% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 or 175 cm2; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 50-70 hours |
| Harvest ： | cell harvest of about 30 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded, hypertriploid karyotype with 10% polyploidy - 71(68-75)<3n>der(X), del(X), -Y, -2, +5, -8, +9, -10, -11, +5mar, add(X)(p21.3), del(X)(q22), add(1)(p36), der(6)t(6;9)(q22.3;q21.1)x2, add(8)(p21), add(9)(p13), del(9)(p21)x2, del(9)(q11), der(11)t(7;11)(q11;p11), add(13)(p12), add(17)(p12) - loss of 9p associated with melanoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |