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| 产品名称： | PSI-2 |
|---|---|
| 商品货号： | TS259232 |
| <strong>Cell line </strong>： | PSI-2 |
| DSMZ no. ： | TS259232 |
| Species ： | originally confirmed as mouse with IEF of AST, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | embryo - NIH-3T3 derivative (retroviral packaging line) |
| Origin ： | subclone of the Swiss mouse embryo cell line NIH-3T3; PSI-2 cells are infected with a replication deficient MLV virus; cells can be used as "packaging cell line"; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14732 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing as monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:10 to 1:20 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 34 hours |
| Harvest ： | cell harvest of about 2-4 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Cytogenetics ： | murine hypotetraploid karyotype - centric fragment present at 74% - ring present at 48% - 68/69(64-74)<4n> - a submetacentric marker arising by centric fusion present at 52% |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |