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| 产品名称： | IGR-37 |
|---|---|
| 商品货号： | TS259235 |
| <strong>Cell line </strong>： | IGR-37 |
| DSMZ no. ： | TS259235 |
| Species ： | originally confirmed as human with IEF of AST, MDH |
| Cell type ： | melanoma |
| Origin ： | established from the lymph node metastasis (groin) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-39 (DSMZ ACC 239); described as strong tumorigenic in nude mice |
| Reference(s) ： | 14363 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing as monolayers; image ; image; image |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS |
| Subculture ： | seed out at ca. 2.5 x 106 cells/75 cm2; split confluent culture 1:2 every 7-14 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca.48-72 hours |
| Harvest ： | cell harvest of about 2-3 x 105/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as IGR-39 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypertriploid karyotype with 11% polyploidy - 78(67-78)<3n>XXY, +1, +3, +5, +7, +7, +8, +9, -10, -12, -13, -14, -15, -17, +18, +20, +20, +2mar, der(1)t(1;?21)(p13;q11)x2, del(9)(p13p21), der(10)t(9;10)(q11/21;q23/25), i(13q)x1-2, der(16)t(1;16)(p11/13;q21/23)x2, der(19)t(17;19)(q12;p13)x1-2 - rearrangements at 1p12/22, 19p13 and +7 recur in malignant melanoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |