微信小李
微信小章
产品简介
购买须知
| 产品名称： | IGR-39 |
|---|---|
| 商品货号： | TS259236 |
| <strong>Cell line </strong>： | IGR-39 |
| DSMZ no. ： | TS259236 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | melanoma |
| Origin ： | established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); same patient as cell line IGR-37 (DSM ACC 237); described as tumorigenic in nude mice |
| Reference(s) ： | 14363 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing in monolayers; image; image |
| Medium ： | 85% Dulbeccos MEM + 15% h.i. FBS |
| Subculture ： | split subconfluent or confluent culture 1:4 every 3-4 days using trypsin/EDTA; seed out at ca. 2 x 106 cells/80 cm2; optimal density of about 4 x 106/175 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | maximal density of about 6-8 x 106/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as IGR-37 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded tetra-/pentaploid karyotype - 104-112<4n>XXY - rearrangements include der(1)t(1;?21)(p13;q11), der(16)t(1;16)(p13;q23) also present in the autologous sister line IGR-37 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |