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| 产品名称： | SAOS-2 |
|---|---|
| 商品货号： | TS259238 |
| <strong>Cell line </strong>： | SAOS-2 |
| DSMZ no. ： | TS259238 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | osteogenic sarcoma |
| Origin ： | established from the primary osteogenic sarcoma of an 11-year-old Caucasian girl in 1973; cells secrete particulate matter containing bone inducing agents thought to comprise bone morphogenic proteins (BMP-1, -2, -3, and -6) |
| Reference(s) ： | 15135, 14538, 14537, 15232 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in monolayers; image ; image |
| Medium ： | 85% McCoys 5A + 15% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2 in 10 ml medium; split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA; cells secrete particulate matter consisting of protein |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-45 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged hyperdiploid karyotype with 16% polyploidy - 56(52-58)<2n>X, -X, -2, -2, +3, -6, -8, +10, -12, +14, +18, -19, +20, -21, -21, -22, +15mar, add(1)(p22), add(3)(q27)x2, add(4)(p15), add(6)(p21), add(7)(p22), add(8)(q24), add(9)(q35), add(11)(p15), add(12)(p13), add(13)(q14), add(14)(p12)x2 - 13q14 breakpoint is associated with osteosarcomas - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |