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| 产品名称： | PB-1 |
|---|---|
| 商品货号： | TS259239 |
| <strong>Cell line </strong>： | PB-1 |
| DSMZ no. ： | TS259239 |
| Species ： | originally confirmed as mouse with IEF of AST and NP and by species PCR and recently as mus musculus by COI DNA barcoding |
| Cell type ： | pre B cells |
| Origin ： | established from the bone marrow of a CBA/C57BL mouse in 1995 |
| Reference(s) ： | 14770 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells are very small and grow mainly in small clusters, but also singly in suspension |
| Medium ： | 85% McCoys 5A + 15% non-heat inactivated FBS + 50 ng/ml human IL-7 + 0.4x MEM vitamins + 1x MEM non-essential amino acids + 0.5x essential amino acids + 1 mM sodium pyruvate + 0.12% sodium bicarbonate + 2 mM L-glutamine + 50 µM mercaptoethanol |
| Subculture ： | cells are difficult to culture! split saturated culture 1:2 every 2-3 days; seed out at ca. 1.0 x 106 cells/ml; after thawing, cells may initially grow rather slowly for about one week; maintain at 1-3 x 106 cells/ml. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | maximum density at ca. 3 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | IL-7 dependency confirmed by 3H-thymidine assay |
| Cytogenetics ： | murine near-diploid karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |