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| 产品名称： | CAPAN-1 |
|---|---|
| 商品货号： | TS259241 |
| <strong>Cell line </strong>： | CAPAN-1 |
| DSMZ no. ： | TS259241 |
| Species ： | confirmed originally as human with IEF of AST, MDH and later by STR fingerprinting |
| Cell type ： | pancreas adenocarcinoma |
| Origin ： | established from the liver metastasis of a pancreatic ductal adenocarcinoma in a 40-year-old Caucasian man in 1974; described as growing in nude mice forming metastases, as resistant to 5-FU, and to express cystic fibrosis transmembrane conductance regulator (CFTR) |
| Reference(s) ： | 15198, 14538, 18731, 18168 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastic semi-confluent monolayer forming loosely adherent foci; image; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split 1:2 to 1:4 every 6-10 days using trypsin/EDTA; after thawing, viability may drop below 60% temporarily and initial growth could be slow |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50-100 hours |
| Harvest ： | cell harvest of about 1.5 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | apparently human karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |