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| 产品名称： | GAMG |
|---|---|
| 商品货号： | TS259244 |
| <strong>Cell line </strong>： | GAMG |
| DSMZ no. ： | TS259244 |
| Species ： | originally confirmed as human with IEF of AST and MDH and recently by STR fingerprinting |
| Cell type ： | glioma |
| Origin ： | established from the biopsy specimens from a 42-year-old woman with glioma (histologically defined as glioblastoma) |
| Reference(s) ： | 14352, 15027 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, large, spindle-like cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 2 mM L-glutamine + 4x MEM non-essential amino acids |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:4 to 1:6 every 4-6 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 17% polyploidy - 66(61-67)<3n>XXX, -4, +5, +7, -10, -13, -19, -22, +dm x1-2, der(1)t(1;4)(q11;p11), del(3)(p14), der(10)t(3;10)(p14;q13), del(17)(p11), del(18)(p11) - additional subclonal rearrangements, add(2)(q11), add(7)(q11), i(9q) - simultaneous +7, -10, -22 and dm recurrent in gliomas |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |