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| 产品名称： | CCRF-CEM |
|---|---|
| 商品货号： | TS259245 |
| <strong>Cell line </strong>： | CCRF-CEM |
| DSMZ no. ： | TS259245 |
| Species ： | confirmed originally as human with IEF of LDH, NP |
| Cell type ： | T cell leukemia |
| Origin ： | established from the peripheral blood of a 3-year-old Caucasian girl with acute lymphoblastic leukemia (ALL) at relapse (terminal) in 1964 (first continuous human T-ALL cell line). Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
| Reference(s) ： | 14541, 15183, 18187 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | growing mainly as adherent epithelioid cells forming a monolayer and to a lower extend as singly round cells in suspension; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells in a 80 cm2 flask; cells grow adherently until confluence as well as in suspension; split confluent culture every 2-4 days; if only suspension cells are used for passaging, a new monolayer will develop; adherent cells should be detached using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24-30 hours |
| Harvest ： | maximal density at ca. 1 x 106 cells/ml (cells in suspension) and 6-8 x 106 cells in 80 cm2 flasks |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD2 -, CD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 -, CD13 -, CD14 -, CD15 +, CD19 -, CD33 -, CD34 -, HLA-DR -, TCRalpha/beta +, TCRgamma/delta -; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-tetraploid karyotype with extensive subclonal variation - 90(88-101)<4n>XX, -X, -X, +20, +20, t(8;9)(p11;p24)x2, der(9)del(9)(p21-22)del(9)(q11q13-21)x2 - sideline with +5, +21, add(13)(q3?3), del(16)(q12) |
| Molec. Genetics ： | expression of NKX2-5 confirmed by RT-PCR and Western blot |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |