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| 产品名称： | OCI-AML5 |
|---|---|
| 商品货号： | TS259249 |
| <strong>Cell line </strong>： | OCI-AML5 |
| DSMZ no. ： | TS259249 |
| Species ： | originally confirmed as human with IEF of AST, LDH, NP and later by STR fingerprinting |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 77-year-old man with acute myeloid leukemia (AML M4) in relapse in 1990; the cells are described in the literature to respond to G-CSF, GM-CSF, IL-3, and FLT3 ligand; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to rapid outgrowth of independent cells |
| Reference(s) ： | 14482, 15051 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | small, round cells growing singly or in clumps in suspension; image; image |
| Medium ： | 70% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637 (DSM ACC 35) (or 10 ng/ml GM-CSF) |
| Subculture ： | seed out at about 0.5 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; optimal split ratio of 1:2 to 1:4 every 2-4 days; viability may decrease after thawing, but cells recover quickly and viability will raise over the next few passages. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | saturation density at about 2.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 -, CD14 -, CD15 +, CD19 -, CD33 +, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype - 48(44-48)<2n>XY, +1, +8, der(1)t(1;19)(p13;p13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |