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| 产品名称： | COLO-677 |
|---|---|
| 商品货号： | TS259252 |
| <strong>Cell line </strong>： | COLO-677 |
| DSMZ no. ： | TS259252 |
| Species ： | originally confirmed as human with IEF of AST, MDH, PEP B; subsequently by DNA fingerprinting |
| Cell type ： | multiple myeloma (derivative of RPMI-8226) |
| Origin ： | originally described in the literature to be derived from the tumor-containing left axillary lymph node of a 39-year-old white man with small lung cell carcinoma (T3 N3 M3) in 1989; however, DNA fingerprinting and cytogenetic analysis showed cross-contamination with cell line RPMI-8226; RPMI-8226 was established from the peripheral blood of a 61-year-old man with multiple myeloma (IgG lambda-type) at diagnosis in 1966 |
| Reference(s) ： | 14749, 14486 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | mostly adherent round cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | harvest of about 16 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as RPMI-8226 by fluorescent nonaplex PCR of short tandem repeat markers |
| Cytogenetics ： | human highly rearranged hypotriploid karyotype with 17% polyploidy - 62(58-62)<3n>XY, -X/Y, -2, -4, -4, -6, -8, -9, -9, -10, -13, -15, -16, +5mar - add(1)(p21), der(3)t(1;3)(q22;q24), add(4)(q32), add(5)(p11), del(7)(q32), add(11)(p14), add(13) (p11)x2, add(14)(q32), i(14q), add(17)(p12) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |