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| 产品名称： | Y-79 |
|---|---|
| 商品货号： | TS259253 |
| <strong>Cell line </strong>： | Y-79 |
| DSMZ no. ： | TS259253 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | retinoblastoma |
| Origin ： | established from the primary tumor (right eye) of a 2-year-old Caucasian girl in 1971 |
| Reference(s) ： | 14888, 14757, 14758 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow in clusters in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + 3.5 g/L glucose + 2 mM L-glutamine |
| Subculture ： | optimal split ratio at 1:2 two to three times per week; seed out at ca.0.25-0.5 x 106 cells/ml; after thawing, viability may drop below 50% temporarily |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40 hours |
| Harvest ： | cell harvest of about 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 10% polyploidy - 47(45-48)<2n>XX, +mar, der(4)t(4;?6)(p15;p21.2), add(12)(q24.32), add(15)(q23), add(20)(q13) - large submetacentric marker with q-arm comprising HSR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |