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| 产品名称： | CAPAN-2 |
|---|---|
| 商品货号： | TS259255 |
| <strong>Cell line </strong>： | CAPAN-2 |
| DSMZ no. ： | TS259255 |
| Species ： | confirmed as human with IEF of AST, MDH, PEP B |
| Cell type ： | pancreas adenocarcinoma |
| Origin ： | established from the tumor of a 56-year-old Caucasian man with pancreas adenocarcinoma in 1975 |
| Reference(s) ： | 14701 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epithelial-like cells growing in islets and uniform monolayers; image; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split semi-confluent (2/3 to 3/4 confluency) culture up to 1:10 every 7 days using trypsin/EDTA (for 10-20 min); adherence may require several hours or overnight |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50-70 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypertriploid karyotype with 6% polyploidy - 68-73<3n>XX, -Y, +1, +1, +4, +5, +7, +11, -12, -14, -17, -18, -22, +3mar - del(1)(p11p33)x2, add(11)(p11), der(11)t(?8;11)(q12;p11)x2, i(14q)x2, add(21)(p11)x2, 1-2DM(24%) - sideline with +1, i(1q)x2, del(1)(q11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |