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| 产品名称： | A-204 |
|---|---|
| 商品货号： | TS259258 |
| <strong>Cell line </strong>： | A-204 |
| DSMZ no. ： | TS259258 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | established from the rhabdomyosarcoma of a 1-year-old girl in 1973; reported to form small malignant tumors in nude mice consistent with embryonic rhabdomyosarcoma |
| Reference(s) ： | 14556 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing in monolayers; image; image |
| Medium ： | 85-90% McCoys 5A + 10-15% h.i. FBS |
| Subculture ： | split confluent culture 1 x per week at up to 1:5 using trypsin/EDTA; seed out directly in a 80 cm2 culture flask, cells will reach confluency within 5-8 days; cells may clump after trypsinization and may be difficult to disperse |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 36 hours |
| Harvest ： | cell harvest of about 5-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near diploid karyotype with tetraploid sideline (31%) and 3.5% polyploidy - 46(41-46)<2n>XX - no consistent chromosome abnormality detected |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |