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| 产品名称： | OMEGA-E |
|---|---|
| 商品货号： | TS259260 |
| <strong>Cell line </strong>： | OMEGA-E |
| DSMZ no. ： | TS259260 |
| Species ： | originally confirmed as mouse with IEF of MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | NIH-3T3 (Swiss mouse embryo) derivative-retroviral packaging cell line |
| Origin ： | derived from NIH-3T3 cells; high titer ecotropic helper-free packaging cell line; generated with separate gagpol and ecotropic env expression constructs with minimal sequence overlap and decreased sequence homology achieved by "codon wobbling"; prior to use in nude mice, cells need to be subcloned; classified as risk category S1 according to the German Central Commission for Biological Safety (ZKBS) |
| Reference(s) ： | 14783 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow as adherent fibroblastic monolayers |
| Medium ： | 90% Dulbeccos MEM + 10% NCS |
| Subculture ： | seed out in a T75 flask and split confluent culture about 1:5 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 20-30 hours |
| Harvest ： | cell harvest of about 5-8 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine hypertriploid karyotype - 71(63-73)<3n> - 1-2 dm (24% cells) |
| Viruses ： | ELISA: reverse transcriptase positive; PCR: SMRV - |