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| 产品名称： | MONO-MAC-1 |
|---|---|
| 商品货号： | TS259262 |
| <strong>Cell line </strong>： | MONO-MAC-1 |
| DSMZ no. ： | TS259262 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | acute monocytic leukemia |
| Origin ： | established from the peripheral blood of a 64-year-old man with acute monocytic leukemia (AML FAB M5) at relapse in 1985 following myeloid metaplasia; simultaneous sister cell line of MONO-MAC-6 (DSM ACC 124); cells are described in the literature to be less differentiated than MONO-MAC-6 and to support productive infection with HIV-1; see also website "www.monocyte.eu". Commercial entities need a usage permit from the original investigator (contact address available from DSMZ; mutz@dsmz.de) |
| Reference(s) ： | 15078, 14554, 14986 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round/multiformed cells in suspension; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine + 1x non-essential amino acids + 1 mM sodium pyruvate |
| Subculture ： | seed out at ca. 1 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio 1:2 to 1:3 every 2-3 days |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-50 hours |
| Harvest ： | maximal density at 1.0-1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 +, CD19 -, CD34 -, cyCD68 +, HLA-DR +; image |
| Fingerprint ： | same DNA profile as MONO-MAC-6 using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near-diploid karyotype with 10% polyploidy - 49(43-52)<2n>XY, +8, +12, +13, +18, -20, inv dup(3)(q21q27), add(8)(q24), t(9;11)(p22;q23), t(10;12;17)(q24;q13;q11), r(12)(??), del(13)(q1?2q21), t(16;21)(q13;q22.2) - diploid version of its sister MONO-MAC-6 with fewer in vitro rearrangements - carries 9;11 translocation (AML M5) |
| Molec. Genetics ： | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |