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| 产品名称： | BEN |
|---|---|
| 商品货号： | TS259263 |
| <strong>Cell line </strong>： | BEN |
| DSMZ no. ： | TS259263 |
| Species ： | originally confirmed as human with IEF of AST, NP |
| Cell type ： | lung carcinoma |
| Origin ： | established from the supraclavicular tumor cells-containing lymph node of a 71-year-old man with poorly differentiated carcinoma of the lung |
| Reference(s) ： | 14507 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid cells growing in monolayers; image; image |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | cell harvest of about 20 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human highly rearranged flat-moded near-tetraploid karyotype - 82-92<4n>X/XY, -X, -Y, -Y, -2, -2, -3, -3, -4, -4, -5, +6, +6, +7, -8, -8, -9, -10, -13, -13, -14, -16, -16, -17, -18, -18, -19, -21, -21, -21, -22, +18mar - del(1)(q22)x2, add(1)(p11), i(2q), i(4q), add(5)(p11), del(6)(q13)x3, del(6)(p21.2), der(7)(t(7;12)(p11;q11), del(7)(q21)x2, i(7p), del(8)(p21), del(9)(p12), del(10)(q24), del(11)(p11)x2, add(12)(q24), i(12q), del(?12)(q14), add(14)(p11), add(18)(q21/23), add(19)(p13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |