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| 产品名称： | DAN-G |
|---|---|
| 商品货号： | TS259264 |
| <strong>Cell line </strong>： | DAN-G |
| DSMZ no. ： | TS259264 |
| Species ： | confirmed originally as human with IEF of AST, NP |
| Cell type ： | pancreas carcinoma |
| Origin ： | established from a human individual with pancreas carcinoma in 1985 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epitheloid cells growing in monolayers; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 33 hours |
| Harvest ： | cell harvest of about 1-3 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human irregular hypotriploid karyotype with 3.5% polyploidy - 59(52-61)<3n>der(X), -X/Y, -X/Y, -1, -2, -3, -4, -5, +6, +7, -9, -10, -13, -16, -17, -18, -20, -22, -22, +4mar, add(2)(p?16), add(4)(q25), i(5p), add(6)(p22), add(7)(p11/13), der(7)t(3;7)(q11;p11), del(7)(p13), add(8)(p11), add(9)(p21), add(12)(p11), i(13q), der(14)t(14;?22)(q32;q12), add(15)(q26), der(19)t(19;hsr)(p13;?), add(20)(q13) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |