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| 产品名称： | COLO-783 |
|---|---|
| 商品货号： | TS259268 |
| <strong>Cell line </strong>： | COLO-783 |
| DSMZ no. ： | TS259268 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | melanoma |
| Origin ： | established in 1990 from the right axillary lymph node of a 48-year-old woman with metastatic malignant melanoma in the right chest |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblasts, not limited to monolayer growth; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | cells grow very slowly; seed out at about 2-3 x 106 cells/80 cm2; split confluent culture 1:2 to 1:3 every 1-2 weeks using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 5-7 days |
| Harvest ： | cell harvest of about 20 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 20% polyloidy - 65(61-69)<3n>XXX, -4, -5, -9, -10, -12, +2mar, add(4)(p11), del(6)(p21), del(7)(p14)x2 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |