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| 产品名称： | HUP-T3 |
|---|---|
| 商品货号： | TS259273 |
| <strong>Cell line </strong>： | HUP-T3 |
| DSMZ no. ： | TS259273 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | pancreas carcinoma |
| Origin ： | established from the malignant ascites of a 66-year-old Japanese man with pancreas carcinoma in 1984 |
| Reference(s) ： | 14808 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently in monolayers and clusters; image |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 1% non-essential amino acids + 1% sodium pyruvate |
| Subculture ： | seed out about 1 x 106 cells/175 cm2; split confluent culture 1:6 to 1:10 once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | about 14 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypodiploid karyotype with 13% polyploidy - 39(36-40)<2n>XY, -6, -8, -9, -10, -12, -13, -17, -17, -19, -20, +3mar (2 rings), del(4)(p15), add(11)(q22.3;HSR), add(12)(q24;HSR), add(19)(p13), add(21)(p12) - gene amplification suggested by large HSR - ch 17 nullisomy |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV - |