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| 产品名称： | LNCAP |
|---|---|
| 商品货号： | TS259276 |
| <strong>Cell line </strong>： | LNCAP |
| DSMZ no. ： | TS259276 |
| Species ： | originally confirmed as human with IEF of AST, MDH and recently by STR-fingerprinting |
| Cell type ： | prostate carcinoma |
| Origin ： | established from the left supraclavicular lymph node metastasis from a 50-year-old man with prostate carcinoma in 1977; cells were described in the literature to be androgen-sensitive |
| Reference(s) ： | 15141, 14557, 14616, 15212 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastoid cells growing in aggregates and as single cells; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/25 cm2; split semi-confluent culture 1:4 once a week using trypsin/EDTA (occasionally cells can be detached by tapping); after thawing and trypsinization cells may need 1-2 days to become adherent again; due to the strong cell aggregation, it might be difficult to determine an exact cell titer |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60 hours |
| Harvest ： | cell harvest of about 10-15 x 106 cells/80 cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotetraploid karyotype with 6% polyploidy - 88(84-93)<4n>XXYY, -2, -4, -6, -6, -13, -13, +2mar, t(1;15)(p22.3;q22.3)x2, del(2)(p16p23), del(10)(q24)x2, der(16)t(6;16)(p22.1;q22)x2 - del(10)(q24) is recurrent in prostate carcinoma - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |