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| 产品名称： | CADO-ES1 |
|---|---|
| 商品货号： | TS259277 |
| <strong>Cell line </strong>： | CADO-ES1 |
| DSMZ no. ： | TS259277 |
| Species ： | originally confirmed as human with IEF of MDH, NP, later by STR fingerprinting |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the malignant pleural effusion from a 19-year-old Japanese woman diagnosed with right-buttock Ewings sarcoma with multiple lung metastases; heterotransplantable; capable of anchorage-independent growth; neural differentiation in response to cAMP |
| Reference(s) ： | 14675, 14676 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round or fusiform cells growing in clumps as adherent or semi-adherent multicellular spheroids; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2-3 x 106 cells in a 80 cm2 flask; split confluent culture 1:4 to 1:10 every 3-7 days using trypsin/EDTA; after thawing use 20% FBS at first |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of about 30 x 106 cells/80cm2 flask |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XX, +8, dup(1)(q32q44), i(8q), add(18)(p11.32) - t(11;22) absent - corresponds to published karyotype - absence of EWS-FLI1 fusion transcripts confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |