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| 产品名称： | COLO-679 |
|---|---|
| 商品货号： | TS259278 |
| <strong>Cell line </strong>： | COLO-679 |
| DSMZ no. ： | TS259278 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | melanoma |
| Origin ： | established in 1985 from the soft tissue metastasis of a malignant melanoma from a 47-year-old woman |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent fibroblastic, but not limited to monolayer growth; image; image |
| Medium ： | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture ： | split confluent culture 1:5 to 1:10 using trypsin/EDTA; change medium twice weekly; seed out at ca. 2 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 34 hours |
| Harvest ： | cell harvest of about 25 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human, flat-moded, hypotetraploid karyotype with 8% polyploidy - 83-92<4n>XXXX, -1, +3, -4, +7, +7, -14, -15, -15, -16, -18, -20, -20, +3mar - del(3)(p11)x1-2, del(3)(q13.3), add(7)(q35)x2, del(9)(p22)x2, add(10)(p11)x1-2, add(10)(p13), add(14)(p11), add(19)(p13.3)x2, add(21)(p11), add(22)(q13)x4 - extensive subclonal variation of chs 1, 5 and 7 - 9p- recurrent in melanoma |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |