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| 产品名称： | TE-671 |
|---|---|
| 商品货号： | TS259281 |
| <strong>Cell line </strong>： | TE-671 |
| DSMZ no. ： | TS259281 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | rhabdomyosarcoma |
| Origin ： | in the original publication and in the ATCC catalogue this cell line is described as "medulloblastoma"; recent studies (Stratton et al., Nature 337: 311-312, 1989; Chen et al., Nature 340: 106, 1989) and the characterization carried out at the DSMZ indicated that TE-671 are muscle cells, most likely identical to the rhabdomyosarcoma cell line RD (also known as TE32 or 130T). |
| Reference(s) ： | 14752 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round and elongated cells with spindle-shape extensions; the round cells contain almost no cytoplasm; cells grow adherent as monolayer; image; image |
| Medium ： | 80-85% Dulbeccos MEM + 15-20% h.i. FBS |
| Subculture ： | seed out about 1 x 106 cells/80 cm2; split culture before cells are entirely confluent, (1:3 to 1:5), using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | 70-80 hours |
| Harvest ： | cell harvest of about 4 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human bimodal hyperdiploid/tetraploid karyotype with 9.7% polyploidy - 43-50/86-98<4n>XX, -X, -X, +1, -2, -4, +7, +8, -9, -9, -13, -14, -14, -15, -15,- 16, -16, -17, -18, -18, -19, -19, -20, -20, -21, -21, +8-11mar, del(1)(p31), add(1)(p31), i(2p), add(3)(p11), add(5)(q35)x2, del(6)(q22)x2, add(9)(q34)x2, add(12)(q24)x2, der(13)t(6;13)(p12.1;p13)x2, add(14)(p11), add(?14)(q24), i(17q), der(19)t(17;19)(q12;p/q13)x2 - ploidy resembles RD embryonal rhabdomyosarcoma of which TE-671 is a subline |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |