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| 产品名称： | LXF-289 |
|---|---|
| 商品货号： | TS259282 |
| <strong>Cell line </strong>： | LXF-289 |
| DSMZ no. ： | TS259282 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | lung adenocarcinoma |
| Origin ： | established from the lung adenocarcinoma of a 63-year-old man |
| Reference(s) ： | 15139 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent epithelial-like cells growing as monolayer; image; image; image; image |
| Medium ： | 90% Hams F10 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 every 3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded near triploid karyotype with 4% polyploidy - 69(62-76)<3n>XY, -X/Y, -1, +6, +9, -13, -14, -18, -19, +22, +3mar, add(1)(p21), i(1q), der(2)t(1;2)(p21;p23)x2, add(4)(p11/15), i(6p), del(12)(q14), i(13q), add(14)(p11), add(15)(p11), del(17)(p11) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |