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| 产品名称： | DU-145 |
|---|---|
| 商品货号： | TS259286 |
| <strong>Cell line </strong>： | DU-145 |
| DSMZ no. ： | TS259286 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | prostate carcinoma |
| Origin ： | established from the tumor tissue removed from the metastatic central nervous system lesion of a 69-year-old man with prostate carcinoma in 1975 |
| Reference(s) ： | 15151, 15188 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayers |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | cell harvest of about 35 x 106 cells/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypotriploid karyotype with 12% polyploidy - 62(58-65)<3n>X, -X/Y, -X/Y, -2, -3, +5, -8, -10, -13, +15, +15, -16, -18, -19, -20, -21, -22, +3mar, del(1)(p31), del(2)(p11), i(5p), del(6)(q22), del(9)(p12), del(11)(q23), der(12)t(11;12)(q11;p11), add(13)(q33), add(13)(q33), add(15)(p11)x2, add(16)(q24) - closely resembles reported karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |