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| 产品名称： | RD-ES |
|---|---|
| 商品货号： | TS259287 |
| <strong>Cell line </strong>： | RD-ES |
| DSMZ no. ： | TS259287 |
| Species ： | originally confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | Ewings sarcoma |
| Origin ： | established from the primary tumor of a 19-year-old Caucasian man with Ewings sarcoma of the humerus in 1984 |
| Reference(s) ： | not published |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent cells growing in monolayers, sometimes growing loosely adherent in clusters/clumps; image |
| Medium ： | 85% RPMI 1640 + 15% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:4 every 5-6 days using trypsin/EDTA (occasional loosely adherent clumps can be detached by tapping the flask); coating the flasks with fibronectin may improve adherence and doubling time |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 60 hours |
| Harvest ： | cell harvest of about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded, near-triploid karyotype - 69(63-72)<3n>XXY/XXYY, +1, -4, -8, +9, +12, +15, -17, +18, -22, der(9)t(9;11)(p13;q11)t(11;22)(q24;q12)x2, add(14)(p11) - carries two masked copies of 11;22 translocation associated with Ewings sarcoma - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |