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| 产品名称： | C-433 |
|---|---|
| 商品货号： | TS259290 |
| <strong>Cell line </strong>： | C-433 |
| DSMZ no. ： | TS259290 |
| Species ： | confirmed as human with IEF of AST, NP |
| Cell type ： | Ewings sarcoma (derivative of RD-ES) |
| Origin ： | thought to be "established from the benign giant cell tumor excised from a woman who had swelling and pain in the right ankle (distal tibia)"; however, DNA fingerprinting and cytogenetic analysis at the DSMZ established unequivocally that this cell line must be considered de facto a subclone of cell line RD-ES (ACC 260) |
| Reference(s) ： | 14827 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | cells grow loosely adherent in monolayers; some cells grow as round or polygonal cells in clumps in suspension with cellular processes |
| Medium ： | 45% RPMI 1640 + 45% McCoys 5A + 10% h.i. FBS |
| Subculture ： | split saturated culture 1:8 to 1:10 every 3-4 days (without using trypsin); seed out at ca. 3-5 x 106 cells/80 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 25-30 hours |
| Harvest ： | cell harvest of about 1.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | same DNA profile as RD-ES using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human flat-moded hypertetraploid karyotype with 4% polyploidy - 103(92-106)<4n>XX, der(X), -X, +1, +1, +1, +1, +2, +6, +6, -14, +17, +18, +6mar, add(X)(p22), del(1)(p22), del(1)(p32p35)x1, der(1)t(1;1)(p32;q11), der(8)t(4;8)(q11;q24), t(9;12)(p12;q13)x2, add(11)(p13), i(13q)x2-3, add(16)(q13), 0-2 dmin/rings present - t(9;12) is associated with benign epithelial tumors - presence of type-2 EWS-FLI1 hybrid transcripts (394 bp) confirmed by RT-PCR |
| Molec. Genetics ： | expression of fusion gene EWS-FLI (type 2) confirmed by RT-PCR |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |