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| 产品名称： | DMBM-2 |
|---|---|
| 商品货号： | TS259292 |
| <strong>Cell line </strong>： | DMBM-2 |
| DSMZ no. ： | TS259292 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, NP, PEP B, and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | macrophages |
| Origin ： | established from the bone marrow of a C3H/HeJ mouse |
| Reference(s) ： | 14778 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing in aggregates, mostly loosely adherent, some cells have long processes |
| Medium ： | 80% RPMI 1640 + 20% horse serum + 1 mM sodium pyruvate + 1 mM non-essential amino acids + 1.25 µg/ml vitamin B12 + 75 µM alpha-thioglycerin + 2 mM L-glutamine + 2.5% 10x L-929 supernatant (DSM ACC 2) |
| Subculture ： | split 1:10 every 4-6 days detaching the cells by flushing the adherent cells with medium through a 25G needle (do not try to desaggregate cells by passing through the needle); cells grow best in Corning round plates (100 x 20 mm) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | cell harvest of about 3-5 x 106 cells/plate |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Cytogenetics ： | murine near-diploid karyotype with 10% tetraploidy - 42(37-43)<2n> - centric fusion markers absent |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |