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| 产品名称： | LAMA-87 |
|---|---|
| 商品货号： | TS259293 |
| <strong>Cell line </strong>： | LAMA-87 |
| DSMZ no. ： | TS259293 |
| Species ： | confirmed as human with IEF of LDH, NP |
| Cell type ： | chronic myeloid leukemia in blast crisis (derivative of LAMA-84) |
| Origin ： | the parental cell line LAMA-84 (DSM ACC 168) has been established from the peripheral blood of a 29-year-old woman with chronic myeloid leukemia (CML) in blast crisis; subclone LAMA-87 was obtained after subcutaneous transplantation of LAMA-84 cells into estrone-treated nude mice; cells are described in the literature to carry t(9;22) leading to BCR-ABL1 e14-a2 (b3-a2) fusion gene |
| Reference(s) ： | 14488, 14479, 14425, 14426 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly or in small clusters in suspension |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 every 2-3 days; spent medium should be exchanged every 24-48 hours even without splitting of the cells; seed out at ca. 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50-60 hours |
| Harvest ： | maximal density at ca. 1 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -; image |
| Fingerprint ： | unique DNA profile using multiplex PCR of minisatellite markers, but identical with LAMA-84 (DSM ACC 168) |
| Cytogenetics ： | human flat-moded hypotriploid karyotype - 60-64<3n>XX, -X, -2, -3, -7, +8, -10, -13, -14, -15, -16, -18, +19, -20, +22, der(9)t(9;22)(q34;q11)x2, del(12)(p12), del(12)(q14), add(19)(p13), add(?19)(p11), der(22)t(9;22)(q34;q11)x4 - four copies of Ph present in most cells - resembles published karyotype (distinguishable from LAMA-84 by 12p- marker) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |