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| 产品名称： | S-117 |
|---|---|
| 商品货号： | TS259295 |
| <strong>Cell line </strong>： | S-117 |
| DSMZ no. ： | TS259295 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | thyroid sarcoma |
| Origin ： | established from the sarcoma of the thyroid gland from a 47-year-old woman in 1985 |
| Reference(s) ： | 15139 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing adherently as monolayer; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | split confluent culture 1:2 about once a week using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 37 hours |
| Harvest ： | cell harvest of about 0.5-1.0 x 105 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hexaploid/octaploid karyotype with 14% polyploidy - 156(140-188)<6n>XXXX, -X, -X - multiple copies of: i(1p), i(1q), i(3p), i(6p), i(9q), add(5)(p15), add(16)(q23), i(mar) - 1-3 dmin/rings in 40% cells - isochromosome formation previously reported in thyroid tumors |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV +, SMRV - |