微信小李
微信小章
产品简介
购买须知
| 产品名称： | AN3-CA |
|---|---|
| 商品货号： | TS259296 |
| <strong>Cell line </strong>： | AN3-CA |
| DSMZ no. ： | TS259296 |
| Species ： | confirmed as human with IEF of AST, MDH, NP |
| Cell type ： | endometrial adenocarcinoma |
| Origin ： | established in 1962 from the lymph node metastasis resected from a 55-year-old white woman with an endometrial adenocarcinoma (epithelial undifferentiated small cell type) presenting with acanthosis nigricans; forms heterotransplantable tumors |
| Reference(s) ： | 14448 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial cells growing as adherent monolayer; prominent cytoplasmic vesicles |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 1 mM sodium pyruvate and non-essential amino acids |
| Subculture ： | split confluent culture 1:3 to 1:8 once or twice weekly using trypsin/EDTA; seed out at ca. 1-2 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 55 hours |
| Harvest ： | cell harvest of about 10-15 x 106 cells/75 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 14% polyploidy - 49(45-50)<2n>XX, +5, +6, +7, der(3)t(1;3)(q24;p22) |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |