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| 产品名称： | CRO-AP3 |
|---|---|
| 商品货号： | TS259299 |
| <strong>Cell line </strong>： | CRO-AP3 |
| DSMZ no. ： | TS259299 |
| Species ： | confirmed as human with IEF of LDH, MDH |
| Cell type ： | B cell lymphoma |
| Origin ： | established from the malignant primary effusion lymphoma (PEL) of a 42-year-old HIV+ man at diagnosis (prior to therapy) without a previous history of Kaposis sarcoma; cells were shown to carry the human herpesvirus 8 (HHV-8), but not the Epstein-Barr virus (EBV) and not the human immunodeficiency virus (HIV) |
| Reference(s) ： | 14418, 14483, 15032 |
| Biosafety level ： | 2 |
| Risk assessment ： | The cell line carries HHV-8 sequences which were detected by PCR and Southern blot. We did not determine the infection mode of this herpes virus and cannot exclude the secretion of the viruses. The cell line is thus categorized biosafety level 2. |
| Permissions and restrictions ： | A, B |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single, round cells growing in suspension; image; image |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture ： | split saturated culture 1:2 to 1:3 every second day; seed out at ca. 0.3 x 106 cells/ml; maintain at ca. 0.2-0.3 x 106 cells/ml; maximal density at ca. 0.6 x 106 cells/ml (maximal density should not be exceeded); initially after thawing, the viability may drop to as low as 30-40% for about 1-2 weeks |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 50 hours |
| Harvest ： | cell harvest of ca. 0.4 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD4 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, cyCD79a -, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda - |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human flat-moded hypotetraploid karyotype - 80-88<4n>XX, -Y, -Y, -1, -2, +6, -7, -10, -13, -13, -22, -22, +6mar, i(1q), add(2)(p2?), add(3)(q2?7), der(4;9)(q10;q10)x1-2, del(5)(p13p15)x1-2, del(5)(q2?1)x1-2, der(6)t(6;7)(q15;q11)add(7)(q3?5), del(8)(p11), del(9)(p21), add(9)(p11), del(11)(q2?2q2?3)x2, der(12)t(2;12)(p12;q24.32), add(13)(p11), t(14;19)(q11;q13)x2, der(15)t(1;?)(?15)(q21;)(?;q11), ider(?)t(8;?)(q11;?) - extensive subclonal rearrangements present - matches published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 +, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |