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| 产品名称： | BF-32 |
|---|---|
| 商品货号： | TS259300 |
| <strong>Cell line </strong>： | BF-32 |
| DSMZ no. ： | TS259300 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and by species PCRand recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgM) reacting with type I and IIA fibers in immunofluorescence and with type 1 and 2A myosin heavy chains (MHCs) in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine fetal muscles |
| Reference(s) ： | 14929 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | grows as single cells or in clumps in suspension; loosely adherent at times |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | split saturated culture at 1:2 to 1:3 every 2-4 days |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 34 hours |
| Harvest ： | maximal density at about 1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypotetraploid karyotype with 4.5% polyploidy - 72-80<4n> - 2-4 dms present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |