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| 产品名称： | B-CPAP |
|---|---|
| 商品货号： | TS259301 |
| <strong>Cell line </strong>： | B-CPAP |
| DSMZ no. ： | TS259301 |
| Species ： | confirmed originally as human with IEF of AST, MDH |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from the tumor tissue of a 76-year-old woman with metastasizing papillary thyroid carcinoma in 1992 |
| Reference(s) ： | 14524, 15201 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle- or round-shaped cells growing adherently as monolayer; image; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:4 to 1:6 every 3-4 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30 hours |
| Harvest ： | about 10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with hexaploid sideline - 70/71(69-72)<3n>XX/XXX, +5, -7, -8, +11, +12, -13, +15, +19, -22, +mar - i(1p), 1qh+, t(5;5)(p13;p15), add(10)(p11), add(12)(p11), del(17)(p11), del(17)(p11)dup(17)(q11q23), add(19)(q13) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |