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| 产品名称： | A-2 |
|---|---|
| 商品货号： | TS259303 |
| <strong>Cell line </strong>： | A-2 |
| DSMZ no. ： | TS259303 |
| Species ： | confirmed Xiphophorus origin by COI DNA Barcoding |
| Cell type ： | non-tumorous embryonal cells |
| Origin ： | derived from non-tumorous epithelial-like embryonal cells of the sweetwater poeciliid fish (platyfish) Xiphophorus xiphidium; origin: Rio Purificacion; strain: YF1 |
| Reference(s) ： | 14695, 14750 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epitheloid cells growing as monolayer |
| Medium ： | 80-85% mixture of Hams F12 + Dulbeccos MEM (at 1:1) + 15-20% h.i. FBS |
| Subculture ： | split culture 1:3 about 2-3 times per week before cells reach confluency; cells grow loosely adherent and can be detached from the plastic surface by shaking or pipetting; old cultures tend to form foci and lose adherence, but the cells remain attached to each other and are difficult to disperse |
| Incubation ： | at 26-28 °C with 10% CO2 |
| Doubling time ： | about 2-3 days |
| Harvest ： | maximal density of 6-10 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Viruses ： | ELISA: reverse transcriptase negative |