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| 产品名称： | MUTZ-2 |
|---|---|
| 商品货号： | TS259304 |
| <strong>Cell line </strong>： | MUTZ-2 |
| DSMZ no. ： | TS259304 |
| Species ： | confirmed as human with IEF of MDH, NP |
| Cell type ： | acute myeloid leukemia |
| Origin ： | established from the peripheral blood of a 62-year-old man with acute myeloid leukemia (AML FAB M2) at diagnosis in 1993; cells are growth factor-dependent and responsive to IL-3, SCF, G-CSF, M-CSF and IFN-gamma |
| Reference(s) ： | 14482, 14618 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single cells in suspension, growing partly in clumps; image; |
| Medium ： | 60% alpha-MEM (with ribo- and deoxyribonucleosides) + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSM ACC 35) (cells grow well with 50 ng/ml SCF) |
| Subculture ： | optimal split ratio of 1:2 to 1:3 twice a week; seed out at about 0.5 x 106 cells/ml; cells should be grown in 24-well-plates. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 47 hours |
| Harvest ： | maximum density at about 1.5-2.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | CD3 -, CD4 +, CD5 -, CD7 -, CD8 -, CD10 -, CD13 +, CD14 -, CD15 +, CD19 -, CD20 -, CD33 +, CD34 +, CD71 +, CD235a -, HLA-DR +; image |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hyperdiploid karyotype with 1.8% polyploidy - 48(46-50)<2n>XY, +8, +10 - no structural aberrations detected - +8 is a typical primary change in AML M1 |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |