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| 产品名称： | BF-G6 |
|---|---|
| 商品货号： | TS259306 |
| <strong>Cell line </strong>： | BF-G6 |
| DSMZ no. ： | TS259306 |
| Species ： | originally confirmed as mouse with IEF of AST, MDH, NP, PEP B and by species PCR and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-rat fiber/myosin) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG1) reacting with human and rat embryonic fibers in immunofluorescence and with human and rat embryonic myosin heavy chains (MHCs) in Western blotting; this antibody reacts more weakly also with adult rat 2B fibers in immunofluorescence and adult rat 2B MHCs in Western blotting; moreover, it stains muscle cells in the conduction tissue of newborn rat heart; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from bovine fetal muscles |
| Reference(s) ： | 14928, 14927, 14572, 14403, 15226 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension (sometimes with loosely adherent cells, sometimes in clumps) |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | optimal split ratio of 1:2 to 1:3 every 2-3 days; seed out at about 0.5 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 30-40 hours |
| Harvest ： | maximal density at about 0.5-1.0 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgG1 monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype - 82(79-88)<4n> - 2-3 dm present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |