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| 产品名称： | PAB-1620 |
|---|---|
| 商品货号： | TS259308 |
| <strong>Cell line </strong>： | PAB-1620 |
| DSMZ no. ： | TS259308 |
| Species ： | originally confirmed as mouse with IEF of LDH and NP and more recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-wild type p53) |
| Origin ： | established in 1982 by immunizing BALB/c mice with VLM tumor cells and fusing splenocytes with SP2/0-Ag14 myeloma cells; described as secreting a mouse monoclonal antibody (IgG2akappa) detecting epitopes of wild type p53 (not mutant or denatured p53), that occur on human, primate, bovine, mouse and rat cells, by ELISA and immunoblotting |
| Reference(s) ： | 14369, 14604 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells growing in suspension, partly slightly adherent |
| Medium ： | 90% Dulbeccos MEM + 10% h.i. FBS + 4 mM L-glutamine |
| Subculture ： | split saturated culture optimally 1:2 to 1:3 every second day; seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3-0.4 x 106 cells/ml; maximal density at ca. 0.7 x 106 cells/ml; some cells attach to the flask surface, but may be detached by tapping the flask; if viability decreases, addition of HFCS ("Hybridoma Fusion and Cloning Supplement") may be useful |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 24 hours |
| Harvest ： | cell harvest of ca. 0.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of IgG2a monoclonal antibody |
| Cytogenetics ： | murine flat-moded hypertetraploid karyotype - 82-97<4n> - several centric fusion markers present |
| Viruses ： | ELISA: reverse transcriptase positive |