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| 产品名称： | 2A1 |
|---|---|
| 商品货号： | TS259310 |
| <strong>Cell line </strong>： | 2A1 |
| DSMZ no. ： | TS259310 |
| Species ： | originally confirmed as mouse with IEF of LDH, NP and recently as mus musculus by COI DNA Barcoding |
| Cell type ： | hybridoma (anti-chicken retina) |
| Origin ： | established by fusion of mouse NS-1 cells with spleen from a BALB/c ByI mouse immunized with White Leghorn chicken embryonic retinal and tectal membranes; secretes 2A1 monoclonal antibody which binds epitopes involved in chicken retinal morphogenesis |
| Reference(s) ： | 14938 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | round cells growing singly in suspension or loosely adherent |
| Medium ： | 80% RPMI 1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing |
| Subculture ： | cells are difficult to culture! maintain at ca. 0.05-0.5 x 106 cells/ml; split saturated culture 1:4 to 1:8 every 2-3 days; seed out at ca. 0.2 x 106 cells/ml |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 25 hours |
| Harvest ： | maximal density at ca. 0.7 x 106 cells/ml (which should not be exceeded) |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | secretion of a IgM monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |