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| 产品名称： | BHT-101 |
|---|---|
| 商品货号： | TS259312 |
| <strong>Cell line </strong>： | BHT-101 |
| DSMZ no. ： | TS259312 |
| Species ： | originally confirmed as human with IEF of AST, G6PD, LDH, NP |
| Cell type ： | thyroid carcinoma |
| Origin ： | established from the lymph node metastasis of a 63-year-old woman with anaplastic papillary thyroid carinoma; cells were described in the literature to not produce hormones, but to be partly positive for thyroglobulin- and thyroxine (T4) |
| Reference(s) ： | 14831 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like cells growing in monolayers; image; image |
| Medium ： | 80% Dulbeccos MEM + 20% h.i. FBS + 0.5% human serum (+ 0.005 IU/ml TSH + 5 µg/ml human insulin - cells grow also without these latter supplements) |
| Subculture ： | seed out ca. 0.5-1 x 106 cells/80cm2; split confluent culture 1:2 to 1:3 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 10% CO2 |
| Doubling time ： | about 2-3 days |
| Harvest ： | cell harvest of 3-4 x 106 cells/80cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 0.5% human serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | apparently human hypertetraploid karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |