微信小李
微信小章
产品简介
购买须知
| 产品名称： | DLD-1 |
|---|---|
| 商品货号： | TS259313 |
| <strong>Cell line </strong>： | DLD-1 |
| DSMZ no. ： | TS259313 |
| Species ： | originally confirmed as human with IEF of MDH, NP and recently by STR fingerprint |
| Cell type ： | colon adenocarcinoma |
| Origin ： | established from a colorectal adenocarcinoma |
| Reference(s) ： | 14456, 15218 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | epithelial-like adherent cells growing as monolayer; image ; image |
| Medium ： | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture ： | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 every 2-3 days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 48 hours |
| Harvest ： | cell harvest of ca. 20 x 106/175 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | identical with HCT-15 (DSM ACC 357), otherwise unique DNA profile using multiplex PCR of minisatellite markers |
| Cytogenetics ： | human near-diploid karyotype with tetraploid (19%) and polyploid sidelines (1.9%) - 46(43-47)<2n>X/XY, add(2)(p13/p23) - corresponds to published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |