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| 产品名称： | DK-MG |
|---|---|
| 商品货号： | TS259315 |
| <strong>Cell line </strong>： | DK-MG |
| DSMZ no. ： | TS259315 |
| Species ： | originally confirmed as human with IEF of AST, LDH, later by STR typing |
| Cell type ： | glioma |
| Origin ： | established from the glioblastoma multiforme (right parietal, at diagnosis) of a 67-year-old woman in 1991 |
| Reference(s) ： | 14689 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | spindle, fibroblast-like cells growing mostly adherent as monolayer; image; image |
| Medium ： | 80-90% RPMI 1640 + 10-20% h.i. FBS + 2 mM L-glutamine |
| Subculture ： | seed out at ca. 1.0 x 106 cells/80 cm2; split nearly confluent culture ca. 1:5 once a week days using trypsin/EDTA |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | ca. 70-80 hours |
| Harvest ： | cell harvest of ca. 4-5 x 106 cells/80 cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human hypodiploid karyotype with 12% polyploidy - 44(42-44)<2n>XX, +7, -9, -10, -12, -14, +mar, del(6)(q13q25), add(9)(p2?2), der(15)t(14;15)(q12;p11), del(16)(p13), del(17)(q25), add(19)(q1?3), variable numbers of dmin detectible in most cells - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV - |