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| 产品名称： | RPMI-2650 |
|---|---|
| 商品货号： | TS259319 |
| <strong>Cell line </strong>： | RPMI-2650 |
| DSMZ no. ： | TS259319 |
| Species ： | confirmed as human with IEF of AST, MDH |
| Cell type ： | nasal septum squamous cell carinoma |
| Origin ： | from the pleural effusion of a 52-year-old man with anaplastic squamous cell carcinoma of the nasal septum in 1962 |
| Reference(s) ： | 14780, 14781 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | adherent, epitheloid, very small cells growing in clusters |
| Medium ： | 90% MEM (with Earles salts) + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture ： | split confluent culture 1:4 to 1:8 every 4-5 days using trypsin/EDTA; seed out at about 0.5-1.0 x 106 cells/25 cm2 |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 40-50 hours |
| Harvest ： | cell harvest of about 0.6 x 106 cells/cm2 |
| Storage ： | frozen with 70% medium, 20% FBS, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology ： | To inquire about expression of EpCAM and intermediate filaments, contact hilmar.quentmeier@dsmz.de. |
| Fingerprint ： | multiplex PCR of minisatellite markers revealed a unique DNA profile |
| Cytogenetics ： | human near-diploid karyotype with 10% polyploidy - 45(43-49)<2n>XY, -4, -16, -18, +2mar, del(1)(p11), add(4)(p16), del(8)(q22), add(14)(p11), add(14)(q32), del(19)(p13) - resembles published karyotype |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/II -, MLV -, SMRV - |