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| 产品名称： | TI-1 |
|---|---|
| 商品货号： | TS259321 |
| <strong>Cell line </strong>： | TI-1 |
| DSMZ no. ： | TS259321 |
| Species ： | originally confirmed as mouse with IEF of AST, LDH, MDH, NP and recently as mus musculus by COI DNA barcoding |
| Cell type ： | hybridoma (anti-rat fiber/troponin I) |
| Origin ： | described as secreting a mouse monoclonal antibody (IgG2b) reacting with rat adult cardiac fibers in immunofluorescence and with rat adult cardiac troponin I in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with myofibrillar preparation from hypothyroid rabbit ventricle |
| Reference(s) ： | 14909 |
| Biosafety level ： | 1 |
| Permissions and restrictions ： | A |
| <strong> </strong>： | DSMZ Scientific Data: |
| Morphology： | single round cells in suspension; culture contains almost always some cell debris and dead cells |
| Medium ： | 90% Dulbeccos MEM (4.5 g/L glucose) + 10% horse serum + 2 mM L-glutamine |
| Subculture ： | split saturated culture at 1:2 to 1:3 every 1-2 days; optimal density at about 0.5-0.8 x 106 cells/ml (not to be cultured below 0.5 x 106 cells/ml) |
| Incubation ： | at 37 °C with 5% CO2 |
| Doubling time ： | about 50 hours |
| Harvest ： | maximal density at about 1.5 x 106 cells/ml |
| Storage ： | frozen with 70% medium, 20% horse serum, 10% DMSO |
| Mycoplasma ： | negative in DAPI, microbiological culture, RNA hybridization assays |
| Immunology ： | secretion of a IgG2b monoclonal antibody |
| Cytogenetics ： | murine hypertetraploid/hypopentaploid karyotype - 92(87-92)<4n>, 1-3 dm present in most cells |
| Viruses ： | ELISA: reverse transcriptase negative; PCR: SMRV - |